VEGFA and neoplasm: To understand the cellular and molecular mechanisms more precisely, we opt for in vitro culture of B16Mel, LLC, and macrophage cells, where we observed significant reduction of VEGF in tumor cells and macrophages after NLGP treatment as early as 4 h at mRNA level with more prominent result in hypoxia than normoxia, suggesting hypoxia as a major factor that NLGP targets.