BRCA2 and breast cancer: Furthermore, although the splicing vector pSAD was initially developed to study the breast cancer genes BRCA1 and BRCA2 (Acedo et al., 2015; Fraile-Bethencourt et al., 2017), it has also been demonstrated to be a powerful tool to test other disease genes such as SERPINA1 (Lara et al., 2014), CHD7 (Villate et al., 2018), and UGT1A1, as well as others currently under investigation3.