Furthermore, although the splicing vector pSAD was initially developed to study the breast cancer genes BRCA1 and BRCA2 (Acedo et al., 2015; Fraile-Bethencourt et al., 2017), it has also been demonstrated to be a powerful tool to test other disease genes such as SERPINA1 (Lara et al., 2014), CHD7 (Villate et al., 2018), and UGT1A1, as well as others currently under investigation3. Here, BRCA1 is linked to breast cancer.