To investigate these hypotheses, a monovalent detergent-split A/Victoria/210/2009 (H3N2; A/Vic) influenza vaccine (0.3 μg/mouse) was adjuvanted with either low dose TLR7/8 agonist (1 μg/mouse) with or without 0.1 μg CRX-601 (1:10 ratio of CRX-601:TLR7/8 agonist), or high dose TLR7/8 agonist (10 μg/mouse) with or without 0.1 μg CRX-601 [1:100 ratio of CRX-601:TLR7/8 agonist; (56]]. Here, TLR7 is linked to influenza.