Here, we build upon these studies by reporting on novel lipidated imidazoquinoline TLR7/8 ligands, alone or in combination with a synthetic TLR4 ligand, and their ability to elicit strong antigen-specific humoral and Th1- or Th17-mediated T cell responses to a co-administered seasonal split H3N2 influenza vaccine in mice. This evidence concerns the gene TLR4 and influenza.