Another single cell analysis of 48 human melanoma samples identified responses to anti-PD-1 to be associated with increased expression of genes coding for TCF7, IL7R, REL, FOXP1, and STAT4 whereas non-response was associated with genes associated with T cell exhaustion, such as CD38, PD-L1, LAG-3, TIM-3, and CTLA-4 (26). This evidence concerns the gene CD274 and melanoma.