These proteins were mainly involved in fatty acid metabolism (Fasn, Scd1, Fads2, Fdps, Plin2), fatty acid degradation (Ehhadh, Acsl1), PPAR signaling pathway (Fads2, Ehhadh, Acsl1, Fabp1), non-alcoholic fatty liver disease (Ehhadh, Pklr) and AMPK signaling pathway (Fasn, Scd1, Hnf4a). The gene discussed is EHHADH; the disease is metabolic dysfunction-associated steatotic liver disease.