DIO has an anti-osteopenic effect on the osteoporosis rat model via attenuating of the RANKL/OPG ratio (Zhang et al., 2014) or regulating long non-coding RNAs (Zhang et al., 2018) in bone tissue, but the effect of DIO on the serum miRNA profile of osteoporosis rats induced by ovariectomy remains unknown. The gene discussed is TNFSF11; the disease is osteoporosis.