TNFSF10 and lung cancer: Additionally, TRAIL-sensitive H460 human lung cancer was used as TRAIL-responsive cancer cells in order to evaluate activity and concentration of TRAIL, and we confirmed activity and concentration (100 ng/ml) of TRAIL is effective and sufficient to induce apoptosis in H460 cells with apoptotic morphology, decreased cell viability and increased cleaved PARP (Fig. 5A,B).