IFNG and infection: To determine the effect of anti-E2 antibody and IFNγ treatment on viral RNA in differentiated (d)AP7 neuronal cells, cells infected with TE-UTR-4Br were imaged from 12 to 72 h after infection using confocal lambda (spectral) mode microscopy that distinguishes the Broccoli signal from autofluorescence (Fig. 3).