IFNG and infection: To confirm these observations, dAP7 cells infected with TEds-10Br, a recombinant double-subgenomic SINV with 6 Broccoli aptamers incorporated downstream of the second subgenomic promoter and another 4 Broccolis incorporated into the 3′UTR, were similarly treated with anti-E2 antibody, IFNγ or anti-TrkA antibody and analyzed by flow cytometry 24 and 48 h after infection (Fig. 4).