Notch pathway targets and effectors were highly induced in our RNA-seq of IMR32 treated with BMP4, so we first validated a panel of Notch pathway genes, including HES1, MAFB, MAML2 and NOTCH3 (Figure 7A), confirming a BMP4-Notch signaling pathway in neuroblastoma. Here, HES1 is linked to neuroblastoma.