PTEN and breast cancer: This analysis also supported the important conclusion that many of the breast cancer driver genes, such as TP53, PIK3CA, PTEN, and BRCA2 can be mutated early or late during breast cancer development: in fact, mutations in TP53, PIK3CA, PTEN, BRCA2, and CDKN2A are fully clonal in some tumors, but subclonal in others [163].