The results of this study showed that the levels of TILs decrease from primary to metastatic disease; a change in gene expression pathways was observed in metastatic tumors with a reduction of immune signatures, particularly a reduction in a signature of interferon gamma signaling; a reduction in some of the T cell subsets and cancer-associated fibroblasts and an increase in endothelial cells and macrophages [540]. This evidence concerns the gene IFNG and metastatic neoplasm.