To confirm that LPS-mediated SAMHD1 activation/dephosphorylation is responsible for block to HIV-1 infection during IFN-independent G0 arrest, we employed SAMHD1 KD in the presence of RUXO to inhibit the effects of any secreted IFN (Figures 3D and 3E). This evidence concerns the gene SAMHD1 and HIV-1 infection.