Compared with participants who did not develop NAFLD, participants who developed NAFLD had older age, lower high-density lipoprotein cholesterol, but higher BMI, WC, total cholesterol, low-density lipoprotein cholesterol, triglycerides, fasting blood glucose, systolic blood pressure, diastolic blood pressure, and alanine aminotransferase (all P <  0.0001). The gene discussed is GPT; the disease is metabolic dysfunction-associated steatotic liver disease.