In the ascites, the proportions of LAG‐3+, PD‐1+, TIM+ and CTLA‐4+ cells in both CD4+ and CD8+ cells were higher than those in the peripheral blood T‐cells.3 Several studies have demonstrated that the regulatory T‐cells (Tregs) and myeloid‐derived suppressor cells (MDSCs) accumulate in the ascites.3, 4, 5 Furthermore, in vitro experiments have demonstrated that the characteristics of cultured immune cells from normal tissue (e.g., peripheral blood from the normal specimen) change upon incubation with the cell‐free ovarian cancer ascites. This evidence concerns the gene CD8A and ovarian cancer.