Herein, we tested whether different ICAM-1 N-glycoforms are present in vivo by modifying the DuoLink® proximity ligation assay (PLA) to detect co-localization of specific N-glycan structures and ICAM-1 in two disease states where endothelial dysfunction has been demonstrated to play a prominent role (see Fig 1 for assay schematic and lectin specificity). The gene discussed is ICAM1; the disease is endothelial dysfunction.