Firstly, we stained tonsil and gallbladder tissue samples and could confirm weak and strong expression, respectively, as described by various protein and RNA expression databases (Figure 2A,B).33, 34 Secondly, we stained five SQCLC cell lines and again could confirm strong expression in H520, H1703, and LK‐2 and minimal expression in H2170 and HCC15 by western blotting and immunocytochemistry (ICC) as described previously (Figure 2C‐H).35, 36, 37 Finally, we knocked out FGFR1 gene in H1703 cell line through targeting exon number 14 using CRISPR/Cas9 system. Here, FGFR1 is linked to intrahepatic cholangiocarcinoma.