To investigate whether PDPK1 and VASP are involved in HOXC10-mediated HCC metastasis, we knocked down PDPK1 and VASP expression in HOXC10-overexpressing Hep3B cells (Hep3B-HOXC10) and ectopically overexpressed PDPK1 and VASP in HCCLM3 cells with HOXC10 knockdown (HCCLM3-shHOXC10) (Figure 3A). The gene discussed is PDPK1; the disease is hepatocellular carcinoma.