Instead, we used a lentiviral vector to establish two CAMSAP2-knockdown cell lines, denoted as “MHCC97H-shCAMSAP2” and “Huh7-shCAMSAP2.” IF staining indicated that MHCC97H cells with high metastatic potential showed an obvious invasion phenotype, whereas MHCC97H-shCAMSAP2 cells formed a spheroidal tumor nest with well-defined borders (Figure 2D). The gene discussed is CAMSAP2; the disease is neoplasm.