TRIO and hepatocellular carcinoma: Bioluminescence imaging of the indicated HCC cells prior to orthotopic implantation is shown in Figure S3A. Consistent with the in-vitro results, EB1 or Trio knockdown markedly lowered the incidence of lung metastasis and prolonged the OS time in the Huh7-Lv-CAMSAP2 group (Figure 4G-H).