The time course of 500 μM DAC uptake and its concentration-dependence at 37 °C for 3 min in 786-O cells are shown in Figure S4A and B. The uptake of DAC was markedly decreased in cells treated with 1 μM NBTI or ENT1 siRNA, indicating that ENT1 was responsible for the uptake of DAC in RCC cells (Figure 4C). The gene discussed is SLC29A1; the disease is renal cell carcinoma.