Importantly, for the CFUS group, the population of functional T cells demonstrated the highest population of CD3+CD8+ and CD4+T cells expressing granzyme B, a key activation marker involved in tumor cell lysis 39 (Figure 6A and B; % Granzyme B+ CD8+ T cells; CFUS: 11.29 ± 3.0, Control: 3.72 ± 0.68, FUS: 3.88 ± 3.40, and CRT-NPs: 4.31 ± 0.97 and for % Granzyme B+ CD4+ T cells; CFUS: 17.85 ± 6.46, Control: 2.82 ± 1.78, FUS: 1.77 ± 0.46, and CRT-NPs 5.01 ± 2.12). Here, GZMB is linked to neoplasm.