Flow cytometry and western blot data suggested that the PD-L1 expressions on tumor cells were not modified by the treatments, but the frequency of PD-L1+ TILs from CFUS tumors was significantly increased (~2.5 folds) compared to control (Figure 7A-C). Additionally, CFUS treatment enhanced the median fluorescence intensity (MFI) of PD-1 on CD3+ CD8+ T cells compared to CRT-NP, control and FUS (Figure 7D). This enhancement was particularly associated with the Granzyme B+ CD8+ T cells (r=0.644, p < 0.01, Figure 7E). The gene discussed is CD274; the disease is neoplasm.