To determine whether Rut selectively overcomes AR-V7 positive prostate cancer cells in vitro, a cell viability analysis was performed using MTS and CCK8 assays in normal prostate stromal (WPMY-1), AR-V7-positive (22Rv1, LNCaP, and C4-2), and AR-V7-negative (PC3 and DU145) cell lines following Rut treatment. This evidence concerns the gene AR and Familial prostate cancer.