To do so, we transduced CF organoids (genotype N1303K/3121-1G > A), which are non-responsive to VX-809 and/or VX-770 (Figure S5), with an LV to stably overexpress 3HA-F508del and assessed functional responses (i.e., CFTR-dependent organoid swelling [12]) to above mentioned modulators. Here, CFTR is linked to cystic fibrosis.