The main problem delaying its generalized use in clinical practice is that first-generation RT-QuIC, performed using recombinant hamster PrP, was unable to detect some specific prions (i.e., variant CJD, the human prion disease acquired from bovine spongiform encephalopathy contaminated sources; some GSS cases due to specific point mutations; etc.)and sensitivity was quite low for other subtypes [19]. Here, PRNP is linked to prion disease.