To validate these findings, we performed real-time PCR assays for four selected lower expressed genes, namely, platelet and endothelial cell adhesion molecule 1 (CD31), matrix metallopeptidase 3 (MMP3), matrix metallopeptidase 9 (MMP9) and vascular endothelial growth factor A (VEGFA) in WT (A375 and MELHO) and RUNT-KO (KO-A375 and KO-MELHO) melanoma cells. The gene discussed is VEGFA; the disease is melanoma.