Taken all together, the evidence from MTT assays, AV-PI and DAPI staining, cell cycle analysis, ROS production, MMP changes and the activation of caspases 3 and 9, demonstrated AC’s promising pro-apoptotic activity towards the human colorectal adenocarcinoma HT-29 cell line via the intrinsic mitochondrial pathway, suggesting that it might be useful as a support to CRC treatment or prevention. Here, CASP3 is linked to colorectal adenocarcinoma.