CD40LG and infection: To evaluate T. muris-specific serum antibody responses induced by vaccination with VLPs+T-cell epitopes, parasite-specific IgM, IgG1, and IgG2c serum antibody levels were determined at d14 p.i. Following vaccination and infection, VLPs+T-cell epitopes and ES/Alum vaccinated mice had statistically significant higher levels of parasite-specific IgM (Fig 3C) and IgG2c (E) compared to the control PBS and native VLP (HBc-Ag) injected mice.