Da Silva Ferreira et al. reported 4/20 ML Flow negative and 3/20 NDO-LID negative (20/20 were PGL-1 ELISA and RLEP PCR positive), and suggested that these false negative lateral flow test results could be related to stage of infection because the false-negative animals showed lower anti-PGL-1 reactivity [22]. This evidence concerns the gene SDHD and infection.