In this study, we tried to differentiate miPSCs into liver CSCs using the CM from HCC cell line Huh7 cells, which exhibited significant expression of liver cancer markers, such as glypican 3, alpha fetoprotein (AFP) and arginase 1 genes, without any genetic manipulation and keeping serial transplantation of primary cultures in the liver. Here, GPC3 is linked to hepatocellular carcinoma.