Our results showed that AKT or NF-κB/p65 could restore the decreased MDR1/P-gp expression induced by CD133 KD, and downregulation of AKT or NF-κB/p65 restored the increased MDR1/P-gp expression induced by CD133 OE in CRC ADR cells. The gene discussed is AKT1; the disease is colorectal carcinoma.