PGP and colorectal carcinoma: The western blot results showed that the MDR1-encoded protein P-gp, p-AKT, p-NF-κB/p65 and CD133 were reduced in MDR CRC cells treated with the CD133 KD plasmid, while total NF-κB/p65 and p-NF-κB/p65 levels were decreased in the nucleus (Fig. 4g).