To determine whether this trend exists across numerous breast cancer cell lines, we analyzed data from the Cancer Cell Line Encyclopedia (CCLE); sorting breast cancer cell lines by EDNRB RNASeq values and analyzing protein expression by RPPA showed a similar trend of increased AKT activation (Fig. 4C); AKT analysis of the 2 available non-cancerous breast cell lines from CCLE by EDNRB expression suggest an opposite pattern, where high EDNRB is associated with lower AKT activation (Fig. 4C). This evidence concerns the gene AKT1 and breast cancer.