To determine whether this trend exists across numerous breast cancer cell lines, we analyzed data from the Cancer Cell Line Encyclopedia (CCLE); sorting breast cancer cell lines by EDNRB RNASeq values and analyzing protein expression by RPPA showed a similar trend of increased AKT activation (Fig. 4C); AKT analysis of the 2 available non-cancerous breast cell lines from CCLE by EDNRB expression suggest an opposite pattern, where high EDNRB is associated with lower AKT activation (Fig. 4C). Here, AKT1 is linked to cancer.