The result of qPCR analysis agreed with the transcriptome sequencing data that RASSF6, DAPK2, CDKN1A, CEACAM1 and TP53I3 were induced in the S6K1-depleted HCC cell lines, and SPK2 and SERBP1 were suppressed under the S6K1 knockdown (Figure 6). This evidence concerns the gene RPS6KB1 and hepatocellular carcinoma.