More importantly, the results of the Ingenuity Pathway Analysis (IPA) in diseases or functions annotation demonstrated that the depletion of SK61 could reduce the tumorigenicity of HCC through the control of hubs of gene including STAT1, HDAC4, CEBPA and ONECUT1 (Figure 4), which is reflected by the inactivation of several cancers including liver tumor (Figure 5 and Table 2, Supplementary Table S8). This evidence concerns the gene HDAC4 and hepatocellular carcinoma.