As shown in Figure 3A and B, mCM and hCM greatly increased the expression of IL-6, IL-1β, IL-1α, TNF-α, IGF-1, and MCP-1 compared with control at the mRNA levels in MCF-7 and MDA-MB-231 cells, while the little effect on the VEGF expression in mCM treated-breast cancer cells. This evidence concerns the gene TNF and breast cancer.