We measured glucose uptake using the 2-NBDG incorporation assay and found that SIX1 overexpression significantly increased the glucose uptake rate in FaDu cells while SIX1 depletion decreased the glucose uptake in Detroit562 cells (Figure 2F).These results indicated that SIX1 was as a key regulator of glucose uptake in HNSCC cells. This evidence concerns the gene SIX1 and head and neck squamous cell carcinoma.