Subsequent applications of real-time quantitative PCR, Western blot, and immunohistochemistry to measure mRNA and protein expression of FRAT1, β-catenin in Wnt signaling transduction in glioma tissues of the tumor-bearing mice indicated that low FRAT1 mRNA and protein expressions in xenograft mice, accompanied with downregulated mRNA and protein expressions of β-catenin. The gene discussed is FRAT1; the disease is central nervous system cancer.