The supernatant of the conditioned medium of MDA-MB-231 cancer cells (MDA-MB-231-EV) had a content of TF-EV (468.90 ± 54.15 pg/mL) significantly higher (p < 0.0001) than that of its control medium (DMEM; not detectable) (Fig. S5). The gene discussed is TF; the disease is cancer.