Thus, to determine whether nano-EGCG has any effects on MMP-2 and MMP-9 in lung cancer cells, we first collected conditioned media of H1299 and CL1-5 cells treated for 24 hours with various concentrations of EGCG or nano-EGCG and analyzed the media through gelatin zymography assay. The gene discussed is MMP9; the disease is lung cancer.