AIF1 and neoplasm: Tumor tissue analyses revealed a significant increase in the number of Iba1+ activated macrophages/microglia despite a minimal change in intratumoural F4/80+ cells (Fig. 5c) in the combination treatment group and was accompanied by increased serum levels of interleukin-2 (IL-2) and tumor necrosis factor alpha (TNFα) (Fig. 5d).