Moreover, PFKFB3 K302A, but not other mutants, significantly abrogated APC/C (Cdc27)-induced PFKFB3 ubiquitination in ESCC cells (Supplementary Fig. 4j, k), further indicating that K302 is an important site for APC/C-mediated ubiquitination in PFKFB3. Here, PFKFB3 is linked to esophageal squamous cell carcinoma.