To establish whether the p53-AGPG-PFKFB3 axis is clinically associated and pathologically involved in ESCC development, we detected AGPG expression by qPCR and Ki67, PFKFB3, CDK1, p27, and p53 expression by IHC in a cohort of ESCC tissues (SYSUCC, n = 102). The gene discussed is PFKFB3; the disease is esophageal squamous cell carcinoma.