Here, we used multiple experimental methods to elucidate whether RUNX1 can effectively reduce NB invasion, metastasis and promote NB-apoptosis by activating baculoviral inhibitor of apoptosis repeat-containing 5 (BIRC5), colony stimulating factor 2 receptor beta (CSF2RB) and NFKBIA. Here, NFKBIA is linked to neuroblastoma.