ACTA1 and metabolic dysfunction-associated steatohepatitis: Our IHC showed that there was remarkably increased α-SMA immunostaining in the fibrotic septa in livers from CDAHFD-fed mice, while little staining of α-SMA in livers from NCD-fed mice; however, there was a relatively weak intensity in livers from myricetin-treated NASH mice when compared that from vehicle-treated NASH mice (Figure 3B).