In the 25 patients without cancer nests outside the capsule (20 patients of Group 1 and five patients of Group 2), there was no abnormal expression of these five proteins in the paratumor liver tissue; CK8, CK19, AFP, and β-catenin were weakly expressed and in the expected locations, and GPC3 was not expressed in the paratumor liver tissue, which clearly indicated the tumor boundary (Figures 6D–F). This evidence concerns the gene AFP and cancer.