GPC3 and cancer: In the 25 patients without cancer nests outside the capsule (20 patients of Group 1 and five patients of Group 2), there was no abnormal expression of these five proteins in the paratumor liver tissue; CK8, CK19, AFP, and β-catenin were weakly expressed and in the expected locations, and GPC3 was not expressed in the paratumor liver tissue, which clearly indicated the tumor boundary (Figures 6D–F).