The positive expression rates of GPC3, CK8, CK19, β-catenin, and AFP in the tumor tissue of these 42 patients were 100% (42/42), 88.1% (37/42), 92.9% (39/42), 92.9% (39/42), and 64.3% (27/42), respectively. The gene discussed is GPC3; the disease is neoplasm.