LC–MS/MS and ELISA-based determination of S1P levels in vitro, from the IC and EC milieu of both infected and uninfected erythrocytes, authenticated that P. falciparum infection drastically reduced the S1P pool (Figure 3), which strongly corroborated our laboratory findings from clinical studies, wherein the S1P level was drastically decreased in uncomplicated and complicated malaria along with thrombocytopenia in P. falciparum- and P. vivax-infected cases (Figure 4A). This evidence concerns the gene MBTPS1 and malaria.