In order to confirm the reduced level of host SphK-1 during parasite infection and its correlation to the decreased phosphorylation pattern, immunoblotting was performed for both SphK-1 and the phosphorylated form of SphK-1 (pSphK-1) in the total cell lysates of parasite-infected and uninfected erythrocytes. Here, SPHK1 is linked to parasitic infectious disease.