In line with this finding, depletion of PFKP resulted in decreased tumor cell migration and invasion in a transwell migration assay (Figure 3E) and a Matrigel invasion assay (Figure 3F); this decrease was abrogated by expression of WT rPFKP, but not rPFKP Y64F (Figures 3E,F). This evidence concerns the gene PFKP and neoplasm.