In the present study, it was shown that HK2 loss downregulated cell growth, EMT-mediated cell movement, and stemness/differentiation-related treatment sensitivity; in addition, altered bioenergetics and modulation of oncogenic signaling molecules including Akt and phosphorylated TP53 could underlie HK2-mediated regulations in HNSCC cells (Figure 5H). This evidence concerns the gene TP53 and head and neck squamous cell carcinoma.