HK2 and head and neck squamous cell carcinoma: As shRNA-mediated HK2-deficient HNSCC cells were successfully established without apparent morphological change (Figures 2B,C and Supplementary Figure 3), it was demonstrated that, by using both trypan blue exclusion (Figure 2D) and MTT (Figure 2E) assays, HK2-silencing resulted in decreased cell growth in HNSCC cells.