HK2 and head and neck squamous cell carcinoma: Numbers of investigations have been reported showing metabolic shift upon HK2 changes in non-HNSCC cells (32, 40, 50, 54); in agreement with these findings, Seahorse analysis showed that HK2 loss led to increased basal and maximal mitochondrial respiration (Figures 5A,B), implying HK2-silencing HNSCC cells were metabolically programmed, being more dependent on mitochondrial metabolism.