Similarly, treatment with PI3K inhibitors suppressed the protein and mRNA levels of PD-L1 and diminished the decreasing/increasing PD-L1 expression effect produced by FGD1 repression/overexpression in osteosarcoma tumor cells, respectively (Figure 6C and 6D, Figure S6D and S6E). The gene discussed is FGD1; the disease is osteosarcoma.