As a biomarker for PMO diagnosis, miR-133a-3p regulates important target genes related to OP, such as VEGFA, SP1, COL1A1, EGFR, etc. These genes are concentrated in key sites of pathways closely related to OP, such as “Osteoarthritis Pathway,” “MAPK signaling pathway,” “TGF-beta signaling pathway,” “Glucocorticoid Receptor Signaling,” “ERK/MAPK Signaling,” etc. In particular, target gene SP1 plays an important role in signal transduction in these pathways. The gene discussed is SP1; the disease is osteoarthritis.