To directly validate the role of STK11 in the pro-adipogenic effect of miR-424 in bovine preadipocytes differentiation, the cells were infected with adenovirus pAd-sh-STK11 and pAd-EGFP, and the green fluorescent protein was analyzed 48 h post-infection to assess the infection efficiency (Figure 4A). This evidence concerns the gene STK11 and infection.