ALCAM and cancer: We performed additional wound-healing assays with other cancer cell lines that do not express endoA3 or CD166 (SUM159 wild type; Supplementary Fig. 10b, g; Supplementary Movie 15), or that express CD166 but not endoA3 (genome-edited clone of SUM159 expressing AP2-GFP; Supplementary Fig. 10c, g; Supplementary Movie 16).