Furthermore, among the 15 patients with loss-of-function of BRCA1/2 (nine tumors with BRCA1 hypermethylation, five tumors with BRCA1 mutation and one tumor with BRCA2 mutation), SigMA identified ten to be Sig3 positive, again consistent with the reported sensitivity of the SigMA algorithm (see methods). This evidence concerns the gene BRCA1 and neoplasm.