SIPA1 and Familial prostate cancer: To analyze the expression of SIPA1 in different cancer cell lines, we determined the mRNA levels of the Sipa1 by quantitative real time-PCR (qRT-PCR) in four breast cancer cell lines (MDA-MB-231, BT549, SK-BR-3 and MCF7), three colon cancer cell lines (HCT116, SW480 and Caco2), two prostate cancer cell lines (PC3 and LNCaP) and an endometrial adenocarcinoma cell line (HEC1A).