TKT and infection: Next, the antiviral activities of Aspergillipeptide D against HSV-1/F and three ACV-resistant strains, including HSV-1/Blue, a TK mutant derived from HSV-1, and two clinical HSV-1 strains HSV-1/106 and HSV-1/153 [19], were monitored by plaque reduction assay (Fig. 2), which clearly showed that Aspergillipeptide D inhibits both HSV-1/F and ACV-resistant strains infection in a dose-dependent manner.